(30. 91% and 47. 22%). Though the number of Merimepodib HIV cases were high, HSV 2 positivity among them was statistically not significant. Conclusion: The purpose of screening for HSV-2 is not only to identify seropositivity, but to help seropositive people identify symptoms and protect themselves Elf3 from acquiring HIV and to protect their partners and seronegative people from acquiring HSV-2 and/or HIV. Keywords: Herpes simplex virus-2, HIV, IgM ELISA, IgG ELISA, Seroprevalence == Introduction == Herpes simplex virus (HSV) infection is one of the most common viral sexually transmitted diseases worldwide, affecting more than one in five sexually active adults with an global estimate of 536 million infected persons and an annual incidence of 23. 6 million cases among persons aged 15 to 49 y [1]. Herpes simplex virus type 2 (HSV-2) infections is considered to be almost exclusively sexually transmitted and may be symptomatic or asymptomatic. condom usage during the sexual intercourses with new partners and with higher number of sexual partners during lifetime. HSV-2 IgM alone was positive in three cases, HSV-2 IgG alone was positive in 26 cases and three had a positive HSV-2 IgM and IgG result. Addition of IgM testing increased rate of detecting seroconversion, 31. 87%, when only IgG ELISA was used, to 35. 16 % patients when IgM test was added. In the study group four cases tested positive for VDRL, and one of them was a known positive case. Among the 55 HIV positive cases in the study group, HSV 2 was positive in 17 cases and among the 36 HIV negative cases HSV 2 was positive in 15 cases. (30. 91% and 47. 22%). Though the number of HIV cases were high, HSV 2 positivity among them was statistically not significant. Conclusion: The purpose of screening for HSV-2 is not only to identify seropositivity, but to help seropositive people identify symptoms and protect themselves from acquiring HIV and to protect their partners and seronegative people from acquiring HSV-2 and/or HIV. Keywords: Herpes simplex virus-2, HIV, IgM ELISA, IgG ELISA, Seroprevalence == Introduction == Herpes simplex virus (HSV) infection is one of the most common viral sexually transmitted diseases worldwide, affecting more than one in five sexually active adults with an Merimepodib global estimate of 536 million infected persons and an annual incidence of 23. 6 million cases among persons aged 15 to 49 y [1]. Herpes simplex virus type 2 (HSV-2) infections is considered to be almost exclusively sexually transmitted and may be symptomatic or asymptomatic. The majority of sexual HSV transmission occurs during asymptomatic periods because the patients are unaware of the asymptomatic virus shedding [2]. The potential role of HSV-2 infection in facilitating HIV transmission highlights the need for including anti-HSV-2 testing and therapy in the management of HIV positive patients, especially for reducing the risk of transmission of HIV through herpetic lesions [3]. The HSV infection may be identified directly by detection of the virus or one of its components or indirectly by assaying for specific serum antibodies of the viruses. After infection with HSV, specific antibodies are formed against the pathogen, which can be detected by various immunological methods. The most significant potential application of serology is to detect the silent carriers of HSV-2, especially in high-risk settings such as STD clinics [4]. The present study was done to evaluate the serological profile of HSV-2 among patients having sexually transmitted infections and to determine the utility of detecting HSV-2 IgM and IgG in such a high- risk group. Merimepodib == Materials and Methods == This prospective study was carried out in the Department of Venerology, Tirunelveli Medical College hospital, TamilNadu, India from April 2013 to September 2013. The study protocol was approved by the ethical committee of the institution. The patients gave written informed consent to participate in the study. == Study population == Patients attending the out Patient clinic of the Department of Venerology of Tirunelveli medical college hospital were taken for study. == Inclusion criteria == All sexually active adults aged 20-49 attending the Outpatient clinic of the Department of Venereology. Patients undergoing treatment for sexually transmitted diseases including HIV. == Exclusion Criteria == 1 . Unwillingness to participate in the study. A predesigned structured Proforma having information on socio-demographic variables such as age, education, occupation, marital status, geographic location, annual income and risk factors that may be associated with sexually transmitted diseases like number of sex partners in previous one year, use of condom, alcohol consumption, commercial sex worker and type of sex partner were recorded. For any cases, the clinical background, signs & symptoms connected with genito-pelvic infections, including earlier history of genital ulcer, pelvic pain, eliminate, pain during sexual intercourse, genital warts, and swelling in the groin likewise recorded. All of the subjects were screened designed for other common sexually transmitted diseases simply by standard lab procedures / commercially available sets. The history and treatment facts of additional sexually transmitted diseases were noted. The HIV status of the individual was recorded after obtaining consent. == Sample collection == By all the 91 patients, 7-8 ml venous blood samples were taken from the brachial blood vessels under aseptic precautions and placed in vacutainers with no anti-coagulant, Serum selections were acquired by centrifuging the specimens for eight min in 3000 rpm. Serums were.